XRN1 Monoclonal Antibody

Catalogue Numbers: MB67147-50, MB67147-100

Sizes: 50µl, 100µl

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q8IZH2

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: 5’-3’ exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover. This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins. XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3’ mRNA fragments generated by RISC cleavage. This process does not require uncapping or deadenylation. XRN1 plays a significant role in viral RNA degradation. As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affect innate immunity and viral replication.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of XRN1 protein.

Extra Notes: Western blot analysis of XRN1 expression in K562 (A), MCF7 (B) whole cell lysates.

Bioworld Molecular Weight: ~ 200 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: SEP1; 5'-3' exoribonuclease 1; Strand-exchange protein 1 homolog

Immunogen: Recombinant fusion protein of human XRN1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified