MGMT Monoclonal Antibody

Catalogue Numbers: MB66904-50, MB66904-100

Sizes: 50µl, 100µl

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P16455

Host: Mouse

Reactivity: Human

Applications: WB, FC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Background: MGMT is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O position of guanine, restoring guanine to its normal form without causing DNA breaks . MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents . It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy .

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MGMT protein.

Extra Notes: Western blot analysis of MGMT expression in Jurkat (A), MCF7 (B), MOLT4 (C), Raji (D) whole cell lysates.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Immunogen: Recombinant fusion protein of human MGMT. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified