ALOX12 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75907-50, BS75907-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P18054

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF,1:50 - 1:200

Background: Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. Mainly converts arachidonate ((5Z,8Z,11Z,14Z-eicosatetraenoate to the specific bioactive lipid (12S-hydroperoxyeicosatetraenoate/(12S-HPETE. Through the production of bioactive lipids like (12S-HPETE it regulates different biological processes including platelet activation. It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S-hydroperoxy-docosahexaenoate/(14S-HPDHA resulting in the formation of (13S,14S-epoxy-DHA. Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoate to generate specialized pro-resolving mediators (SPMs like resolvin D5 ((7S,17S-diHPDHA and (7S,14S-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 76KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of A-431, using ALOX12 antibody at 1:1140 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded rat heart using ALOX12 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human colon carcinoma using ALOX12 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using ALOX12 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: ALOX12; 12-LOX; 12S-LOX; LOG12; 12S-type

Immunogen: Recombinant fusion protein of human ALOX12(NP_000688.2).

Conjugate: Unconjugated

Modification: Unmodified