Nucleophosmin Monoclonal Antibody

Catalogue Numbers: MB66482-50, MB66482-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P06748

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, ChIP

All Applications: WB (1/500 - 1/1000), ChIP (1/10 - 1/50)

Background: Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Nucleophosmin protein.

Extra Notes: Western blot analysis of Nucleophosmin expression in Hela (A), Jurkat (B), NIH3T3 (C) whole cell lysates.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

Immunogen: Purified recombinant full length of human NPM1 protein expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified